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stat5  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc stat5
    Stat5, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 552 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stat5/Stat5+Rabbit+mAb/pm42830359-233-32-36
    Average 96 stars, based on 552 article reviews
    stat5 - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Targeting the CD4 + effector memory T cells re-expressing CD45RA suppressor galectin-7: a multi-omics-guided therapeutic strategy for idiopathic pulmonary fibrosis.
    Article Snippet: .. After blocking with 5% non-fat milk in TBST for 1 h at room temperature, membranes were incubated overnight at 4 °C with primary antibodies against p-STAT5 (Tyr694, Catalog # 9314S, CST, 1:1000), STAT5 (Catalog # 94,205, CST, 1:1000), p-JAK1 (Tyr1034/1035, Catalog # 74129S, CST, 1:1000), JAK1 (Catalog # 3344S, CST, 1:1000), p-JAK3 (Tyr980/981, Catalog # 5031S, CST, 1:1000), JAK3 (Catalog # 8827S, CST, 1:1000), IL-7Rα/CD127 (Catalog # 17,626–1-AP, Proteintech, 1:1000), and β-actin (Catalog # GB15001, Servicebio, 1:5000) as loading control. .. Membranes were then incubated with HRP-conjugated goat anti-mouse IgG (Catalog # SA00001-1, Proteintech, 1:5000) or HRPconjugated goat anti-rabbit IgG (Catalog # SA00001-2, Proteintech, 1:5000).

    Incubation:

    Article Title: Targeting the CD4 + effector memory T cells re-expressing CD45RA suppressor galectin-7: a multi-omics-guided therapeutic strategy for idiopathic pulmonary fibrosis.
    Article Snippet: .. After blocking with 5% non-fat milk in TBST for 1 h at room temperature, membranes were incubated overnight at 4 °C with primary antibodies against p-STAT5 (Tyr694, Catalog # 9314S, CST, 1:1000), STAT5 (Catalog # 94,205, CST, 1:1000), p-JAK1 (Tyr1034/1035, Catalog # 74129S, CST, 1:1000), JAK1 (Catalog # 3344S, CST, 1:1000), p-JAK3 (Tyr980/981, Catalog # 5031S, CST, 1:1000), JAK3 (Catalog # 8827S, CST, 1:1000), IL-7Rα/CD127 (Catalog # 17,626–1-AP, Proteintech, 1:1000), and β-actin (Catalog # GB15001, Servicebio, 1:5000) as loading control. .. Membranes were then incubated with HRP-conjugated goat anti-mouse IgG (Catalog # SA00001-1, Proteintech, 1:5000) or HRPconjugated goat anti-rabbit IgG (Catalog # SA00001-2, Proteintech, 1:5000).

    Control:

    Article Title: Targeting the CD4 + effector memory T cells re-expressing CD45RA suppressor galectin-7: a multi-omics-guided therapeutic strategy for idiopathic pulmonary fibrosis.
    Article Snippet: .. After blocking with 5% non-fat milk in TBST for 1 h at room temperature, membranes were incubated overnight at 4 °C with primary antibodies against p-STAT5 (Tyr694, Catalog # 9314S, CST, 1:1000), STAT5 (Catalog # 94,205, CST, 1:1000), p-JAK1 (Tyr1034/1035, Catalog # 74129S, CST, 1:1000), JAK1 (Catalog # 3344S, CST, 1:1000), p-JAK3 (Tyr980/981, Catalog # 5031S, CST, 1:1000), JAK3 (Catalog # 8827S, CST, 1:1000), IL-7Rα/CD127 (Catalog # 17,626–1-AP, Proteintech, 1:1000), and β-actin (Catalog # GB15001, Servicebio, 1:5000) as loading control. .. Membranes were then incubated with HRP-conjugated goat anti-mouse IgG (Catalog # SA00001-1, Proteintech, 1:5000) or HRPconjugated goat anti-rabbit IgG (Catalog # SA00001-2, Proteintech, 1:5000).

    Article Title: Combining lenalidomide with IL-2 family of cytokines enhances activating receptor and perforin/granzyme expression in NK cells
    Article Snippet: After assessing that the highest band transferred completely membranes were retrieved and blocked with 5% nonfat milk-based tris buffer with 0.05% Tween 20 (cat# BP337−500, Thermo Fisher Scientific, Waltham, Massachusetts, United States). .. The primary antibodies used for protein expression assessment were: total STAT5 (D3N2B, 1:1000, cat# 25656, Cell Signaling Technology, Danvers, Massachusetts, United States, RRID:AB_2798908), phospho- STAT5 (C11C5, 1:1000, cat# 9359P, Cell Signaling Technology, Danvers, Massachusetts, United States), total AKT (11E7, 1:1000, cat# 4685S, Cell Signaling Technology, Danvers, Massachusetts, United States), phospho-AKT (D25E6, 1:1000, cat# 130385, Cell Signaling Technology, Danvers, Massachusetts, United States), PI3K p85 (I9H8, 1:1000, cat# 4257, Cell Signaling Technology, Danvers, Massachusetts, United States, RRID:AB_659889) and beta-actin as a loading control (AC-74, 1:5000, cat#A5316, Sigma-Aldrich , St. Louis, Missouri, United States, RRID:AB_476743). ..

    Article Title: Combining lenalidomide with IL-2 family of cytokines enhances activating receptor and perforin/granzyme expression in NK cells.
    Article Snippet: After assessing that the highest band transferred completely membranes were retrieved and blocked with 5% nonfat milk-based tris buffer with 0.05% Tween 20 (cat# BP337−500, Thermo Fisher Scientific, Waltham, Massachusetts, United States). .. The primary antibodies used for protein expression assessment were: total STAT5 (D3N2B, 1:1000, cat# 25656, Cell Signaling Technology, Danvers, Massachusetts, United States, RRID:AB_2798908), phospho- STAT5 (C11C5, 1:1000, cat# 9359P, Cell Signaling Technology, Danvers, Massachusetts, United States), total AKT (11E7, 1:1000, cat# 4685S, Cell Signaling Technology, Danvers, Massachusetts, United States), phospho-AKT (D25E6, 1:1000, cat# 130385, Cell Signaling Technology, Danvers, Massachusetts, United States), PI3K p85 (I9H8, 1:1000, cat# 4257, Cell Signaling Technology, Danvers, Massachusetts, United States, RRID:AB_659889) and beta-actin as a loading control (AC-74, 1:5000, cat#A5316, Sigma-Aldrich, St. Louis, Missouri, United States, RRID:AB_476743). ..

    Western Blot:

    Article Title: Sirtuin 1 Inhibition Attenuates Viral Myocarditis by Suppressing the Nuclear Translocation of Pyruvate Kinase M2 to Rebalance Th17/Th9 Differentiation
    Article Snippet: .. The Western blotting antibodies used targeted following proteins: SIRT1 (1:1000, catalog: 2028, CST, USA), PKM2 (1:1000, catalog:15822-1-AP, Proteintech, China), ALDOA (1:5000, catalog: 11217-1-AP, Proteintech, China), HK2 (1:5000, catalog: 22029-1-AP, Proteintech, China), PFKFB1 (1:1000, catalog: 21718-1-AP, Proteintech, China), GLUT1 (1:2000, catalog: A11208, Abclonal, China), STAT3 (1:1000, catalog: 12640, CST, USA), phospho-STAT3 (1:1000, catalog: 9145, CST, USA), STAT5 (1:1000, catalog: 94205, CST, USA), phospho-STAT5 (1:1000, catalog: 9359, CST, USA), RORγt (1:1000, catalog:ab207082, abcam, UK), PU.1 (1:1000, catalog: 2258, CST, USA), Foxp3 (1:500, catalog:85128-6-RR, Proteintech, China), β-actin (1:3000, catalog: ANT321, Antgene, China), GAPDH (1:5000, catalog: 60004-1-Ig, Proteintech, China), and H3 (1:2000, catalog:17168-1-AP, Proteintech, China). .. Following primary antibody incubation, the membranes were probed with respective anti-rabbit or antimouse HRP-conjugated secondary antibodies (1:3000, AntGene, China) for 2 hours at room temperature and visualized using the electrochemiluminescence (ECL) kit (Thermo Scientific, USA).

    Article Title: CXCL5 suppresses osteoclastogenesis and protects against lipoteichoic acid-induced bone loss by modulating PLCγ2 and c-Fos signalling in gram-positive periprosthetic joint infection.
    Article Snippet: Protein concentrations were quantified using the BCA Protein Assay Kit (Thermo Fisher Scientific). .. Western blotting was performed using primary antibodies against the following targets: nuclear factor of activated T-cells cytoplasmic 1 (NFATc1) (4389), phospho phospholipase Cγ2 (PLCγ2) (Tyr1217, 3871T), PLCγ2 (3872T), phospho-PLCγ1 (Tyr783, 2821T), PLCγ1 (5690T), c-Fos (4384), calcineurin A (2614S), phospho-p38 mitogen-activated protein kinase (MAPK) (Thr180/Tyr182, 9211), p38 MAPK (8690), phospho-SAPK/JNK (Thr183/Tyr185, 4668), SAPK/JNK (9252), phospho-ERK1/2 (4370), ERK1/2 (9102), phospho-nuclear factor-kappa B (NF-κB) p65 (Ser536, 3033), NF-κB p65 (8242), phospho-signal transducer and activator of transcription 5 (STAT5) (Tyr694, 9359S), STAT5 (94205T), phospho-STAT6 (Tyr641, 56554S), STAT6 (5397S), phospho-FAK (Tyr397, 3283S), and FAK (3285T) (all from Cell Signaling Technology, USA); TRAP (ab191406) and CXCR2 (ab217314; Abcam); CTSK (sc-48353), tumour necrosis factor receptor-associated factor 6 (TRAF6; sc-8409), and β-actin (sc-47778; Santa Cruz Biotech nology, USA). .. Western blotting was performed using primary antibodies against the following targets: nuclear factor of activated T-cells cytoplasmic 1 (NFATc1) (4389), phospho phospholipase Cγ2 (PLCγ2) (Tyr1217, 3871T), PLCγ2 (3872T), phospho-PLCγ1 (Tyr783, 2821T), PLCγ1 (5690T), c-Fos (4384), calcineurin A (2614S), phospho-p38 mitogen-activated protein kinase (MAPK) (Thr180/Tyr182, 9211), p38 MAPK (8690), phospho-SAPK/JNK (Thr183/Tyr185, 4668), SAPK/JNK (9252), phospho-ERK1/2 (4370), ERK1/2 (9102), phospho-nuclear factor-kappa B (NF-κB) p65 (Ser536, 3033), NF-κB p65 (8242), phospho-signal transducer and activator of transcription 5 (STAT5) (Tyr694, 9359S), STAT5 (94205T), phospho-STAT6 (Tyr641, 56554S), STAT6 (5397S), phospho-FAK (Tyr397, 3283S), and FAK (3285T) (all from Cell Signaling Technology, USA); TRAP (ab191406) and CXCR2 (ab217314; Abcam); CTSK (sc-48353), tumour necrosis factor receptor-associated factor 6 (TRAF6; sc-8409), and β-actin (sc-47778; Santa Cruz Biotech nology, USA).

    Article Title: Activating mutations in ESR1 contribute to an immunosuppressive breast tumor microenvironment by dampening cytokine secretion.
    Article Snippet: Flash-frozen mammary gland or tumor pieces were crushed in liquid nitrogen, and cells were collected into Eppendorf tubes by scraping and rinsed once in 1X PBS. .. The following antibodies were used for immunoblots: β-actin (Sigma, A5441, 1:2000), tubulin (Cell Signaling Technology (CST), 2148, 1:1000), Cyclin D1 (CST, 2922, 1:500), FOXA1 (Abcam, ab23738, 1:1000), Stat3 (CST, 9139, 1:1000), Stat5 (CST, 94205, 1:1000), p-Stat3 (CST, 9145, 1:1000), and p-Stat5 (CST, 4322, 1:500). ..

    Article Title: CXCL5 suppresses osteoclastogenesis and protects against lipoteichoic acid-induced bone loss by modulating PLCγ2 and c-Fos signalling in gram-positive periprosthetic joint infection
    Article Snippet: .. Western blotting was performed using primary antibodies against the following targets: nuclear factor of activated T-cells cytoplasmic 1 (NFATc1) (4389), phospho-phospholipase Cγ2 (PLCγ2) (Tyr1217, 3871T), PLCγ2 (3872T), phospho-PLCγ1 (Tyr783, 2821T), PLCγ1 (5690T), c-Fos (4384), calcineurin A (2614S), phospho-p38 mitogen-activated protein kinase (MAPK) (Thr180/Tyr182, 9211), p38 MAPK (8690), phospho-SAPK/JNK (Thr183/Tyr185, 4668), SAPK/JNK (9252), phospho-ERK1/2 (4370), ERK1/2 (9102), phospho-nuclear factor-kappa B (NF-κB) p65 (Ser536, 3033), NF-κB p65 (8242), phospho-signal transducer and activator of transcription 5 (STAT5) (Tyr694, 9359S), STAT5 (94205T), phospho-STAT6 (Tyr641, 56554S), STAT6 (5397S), phospho-FAK (Tyr397, 3283S), and FAK (3285T) (all from Cell Signaling Technology, USA); TRAP (ab191406) and CXCR2 (ab217314; Abcam); CTSK (sc-48353), tumour necrosis factor receptor-associated factor 6 (TRAF6; sc-8409), and β-actin (sc-47778; Santa Cruz Biotechnology, USA). .. Signal detection was carried out using enhanced chemiluminescence solution (Revvity, USA) and visualized with a UVP imaging system (Analytik Jena, Germany).

    Cell-Signaling:

    Article Title: CXCL5 suppresses osteoclastogenesis and protects against lipoteichoic acid-induced bone loss by modulating PLCγ2 and c-Fos signalling in gram-positive periprosthetic joint infection
    Article Snippet: .. Western blotting was performed using primary antibodies against the following targets: nuclear factor of activated T-cells cytoplasmic 1 (NFATc1) (4389), phospho-phospholipase Cγ2 (PLCγ2) (Tyr1217, 3871T), PLCγ2 (3872T), phospho-PLCγ1 (Tyr783, 2821T), PLCγ1 (5690T), c-Fos (4384), calcineurin A (2614S), phospho-p38 mitogen-activated protein kinase (MAPK) (Thr180/Tyr182, 9211), p38 MAPK (8690), phospho-SAPK/JNK (Thr183/Tyr185, 4668), SAPK/JNK (9252), phospho-ERK1/2 (4370), ERK1/2 (9102), phospho-nuclear factor-kappa B (NF-κB) p65 (Ser536, 3033), NF-κB p65 (8242), phospho-signal transducer and activator of transcription 5 (STAT5) (Tyr694, 9359S), STAT5 (94205T), phospho-STAT6 (Tyr641, 56554S), STAT6 (5397S), phospho-FAK (Tyr397, 3283S), and FAK (3285T) (all from Cell Signaling Technology, USA); TRAP (ab191406) and CXCR2 (ab217314; Abcam); CTSK (sc-48353), tumour necrosis factor receptor-associated factor 6 (TRAF6; sc-8409), and β-actin (sc-47778; Santa Cruz Biotechnology, USA). .. Signal detection was carried out using enhanced chemiluminescence solution (Revvity, USA) and visualized with a UVP imaging system (Analytik Jena, Germany).

    Expressing:

    Article Title: Combining lenalidomide with IL-2 family of cytokines enhances activating receptor and perforin/granzyme expression in NK cells
    Article Snippet: After assessing that the highest band transferred completely membranes were retrieved and blocked with 5% nonfat milk-based tris buffer with 0.05% Tween 20 (cat# BP337−500, Thermo Fisher Scientific, Waltham, Massachusetts, United States). .. The primary antibodies used for protein expression assessment were: total STAT5 (D3N2B, 1:1000, cat# 25656, Cell Signaling Technology, Danvers, Massachusetts, United States, RRID:AB_2798908), phospho- STAT5 (C11C5, 1:1000, cat# 9359P, Cell Signaling Technology, Danvers, Massachusetts, United States), total AKT (11E7, 1:1000, cat# 4685S, Cell Signaling Technology, Danvers, Massachusetts, United States), phospho-AKT (D25E6, 1:1000, cat# 130385, Cell Signaling Technology, Danvers, Massachusetts, United States), PI3K p85 (I9H8, 1:1000, cat# 4257, Cell Signaling Technology, Danvers, Massachusetts, United States, RRID:AB_659889) and beta-actin as a loading control (AC-74, 1:5000, cat#A5316, Sigma-Aldrich , St. Louis, Missouri, United States, RRID:AB_476743). ..

    Article Title: Combining lenalidomide with IL-2 family of cytokines enhances activating receptor and perforin/granzyme expression in NK cells.
    Article Snippet: After assessing that the highest band transferred completely membranes were retrieved and blocked with 5% nonfat milk-based tris buffer with 0.05% Tween 20 (cat# BP337−500, Thermo Fisher Scientific, Waltham, Massachusetts, United States). .. The primary antibodies used for protein expression assessment were: total STAT5 (D3N2B, 1:1000, cat# 25656, Cell Signaling Technology, Danvers, Massachusetts, United States, RRID:AB_2798908), phospho- STAT5 (C11C5, 1:1000, cat# 9359P, Cell Signaling Technology, Danvers, Massachusetts, United States), total AKT (11E7, 1:1000, cat# 4685S, Cell Signaling Technology, Danvers, Massachusetts, United States), phospho-AKT (D25E6, 1:1000, cat# 130385, Cell Signaling Technology, Danvers, Massachusetts, United States), PI3K p85 (I9H8, 1:1000, cat# 4257, Cell Signaling Technology, Danvers, Massachusetts, United States, RRID:AB_659889) and beta-actin as a loading control (AC-74, 1:5000, cat#A5316, Sigma-Aldrich, St. Louis, Missouri, United States, RRID:AB_476743). ..

    other:

    Article Title: Activating mutations in ESR1 contribute to an immunosuppressive breast tumor microenvironment by dampening cytokine secretion
    Article Snippet: Band intensity quantification was done using Image Studio Lite software (Li-Cor).



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