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phospho stat5  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc phospho stat5
    Phospho Stat5, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 335 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stat5/Phospho-Stat5+(Tyr694)+XP+Rabbit+mAb/pmc13049607-338-59-61
    Average 96 stars, based on 335 article reviews
    phospho stat5 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Membrane:

    Article Title: Enhancement of NK cell activity via DNA-sensing inhibition by Poxin transgene
    Article Snippet: Protein concentrations were determined with the BCA Protein Assay Kit (Thermo Fisher). .. Proteins were then transferred onto a PVDF membrane, blocked with PBST buffer containing 5 % skim milk for 1 h at room temperature, and incubated overnight at 4 °C with primary antibodies, including phospho-STING (Ser366) (#19781, Cell Signaling Technology (CST)), STING (#13647, CST), phospho-TBK1 (Ser172) (#5483, CST), TBK1 (#3504, CST), phospho-IRF3 (S386) (ab76493, Abcam), IRF3 (#4302, CST), phospho-P53 (Ser15) (#9284, CST), Bcl-xL (#2764, CST), GAPDH (#5174, CST), GFP (sc-9996, Santa Cruz), phospho-NF-κB-P65 (Ser536) (#3033, CST), NF-κB-P65 (#8242, CST), Histone H2A.X (#7631, CST), phospho-JAK1 (Tyr1034/1035) (#3331, CST), JAK1 (#3344, CST), phospho-STAT5 (Tyr694) (#9359, CST), and STAT5 (#9363, CST). .. Proteins were then transferred onto a PVDF membrane, blocked with PBST buffer containing 5 % skim milk for 1 h at room temperature, and incubated overnight at 4 °C with primary antibodies, including phospho-STING (Ser366) (#19781, Cell Signaling Technology (CST)), STING (#13647, CST), phospho-TBK1 (Ser172) (#5483, CST), TBK1 (#3504, CST), phospho-IRF3 (S386) (ab76493, Abcam), IRF3 (#4302, CST), phospho-P53 (Ser15) (#9284, CST), Bcl-xL (#2764, CST), GAPDH (#5174, CST), GFP (sc-9996, Santa Cruz), phospho-NF-κB-P65 (Ser536) (#3033, CST), NF-κB-P65 (#8242, CST), Histone H2A.X (#7631, CST), phospho-JAK1 (Tyr1034/1035) (#3331, CST), JAK1 (#3344, CST), phospho-STAT5 (Tyr694) (#9359, CST), and STAT5 (#9363, CST).

    Incubation:

    Article Title: Enhancement of NK cell activity via DNA-sensing inhibition by Poxin transgene
    Article Snippet: Protein concentrations were determined with the BCA Protein Assay Kit (Thermo Fisher). .. Proteins were then transferred onto a PVDF membrane, blocked with PBST buffer containing 5 % skim milk for 1 h at room temperature, and incubated overnight at 4 °C with primary antibodies, including phospho-STING (Ser366) (#19781, Cell Signaling Technology (CST)), STING (#13647, CST), phospho-TBK1 (Ser172) (#5483, CST), TBK1 (#3504, CST), phospho-IRF3 (S386) (ab76493, Abcam), IRF3 (#4302, CST), phospho-P53 (Ser15) (#9284, CST), Bcl-xL (#2764, CST), GAPDH (#5174, CST), GFP (sc-9996, Santa Cruz), phospho-NF-κB-P65 (Ser536) (#3033, CST), NF-κB-P65 (#8242, CST), Histone H2A.X (#7631, CST), phospho-JAK1 (Tyr1034/1035) (#3331, CST), JAK1 (#3344, CST), phospho-STAT5 (Tyr694) (#9359, CST), and STAT5 (#9363, CST). .. Proteins were then transferred onto a PVDF membrane, blocked with PBST buffer containing 5 % skim milk for 1 h at room temperature, and incubated overnight at 4 °C with primary antibodies, including phospho-STING (Ser366) (#19781, Cell Signaling Technology (CST)), STING (#13647, CST), phospho-TBK1 (Ser172) (#5483, CST), TBK1 (#3504, CST), phospho-IRF3 (S386) (ab76493, Abcam), IRF3 (#4302, CST), phospho-P53 (Ser15) (#9284, CST), Bcl-xL (#2764, CST), GAPDH (#5174, CST), GFP (sc-9996, Santa Cruz), phospho-NF-κB-P65 (Ser536) (#3033, CST), NF-κB-P65 (#8242, CST), Histone H2A.X (#7631, CST), phospho-JAK1 (Tyr1034/1035) (#3331, CST), JAK1 (#3344, CST), phospho-STAT5 (Tyr694) (#9359, CST), and STAT5 (#9363, CST).

    Western Blot:

    Article Title: CXCL5 suppresses osteoclastogenesis and protects against lipoteichoic acid-induced bone loss by modulating PLCγ2 and c-Fos signalling in gram-positive periprosthetic joint infection.
    Article Snippet: Protein concentrations were quantified using the BCA Protein Assay Kit (Thermo Fisher Scientific). .. Western blotting was performed using primary antibodies against the following targets: nuclear factor of activated T-cells cytoplasmic 1 (NFATc1) (4389), phospho phospholipase Cγ2 (PLCγ2) (Tyr1217, 3871T), PLCγ2 (3872T), phospho-PLCγ1 (Tyr783, 2821T), PLCγ1 (5690T), c-Fos (4384), calcineurin A (2614S), phospho-p38 mitogen-activated protein kinase (MAPK) (Thr180/Tyr182, 9211), p38 MAPK (8690), phospho-SAPK/JNK (Thr183/Tyr185, 4668), SAPK/JNK (9252), phospho-ERK1/2 (4370), ERK1/2 (9102), phospho-nuclear factor-kappa B (NF-κB) p65 (Ser536, 3033), NF-κB p65 (8242), phospho-signal transducer and activator of transcription 5 (STAT5) (Tyr694, 9359S), STAT5 (94205T), phospho-STAT6 (Tyr641, 56554S), STAT6 (5397S), phospho-FAK (Tyr397, 3283S), and FAK (3285T) (all from Cell Signaling Technology, USA); TRAP (ab191406) and CXCR2 (ab217314; Abcam); CTSK (sc-48353), tumour necrosis factor receptor-associated factor 6 (TRAF6; sc-8409), and β-actin (sc-47778; Santa Cruz Biotech nology, USA). .. Western blotting was performed using primary antibodies against the following targets: nuclear factor of activated T-cells cytoplasmic 1 (NFATc1) (4389), phospho phospholipase Cγ2 (PLCγ2) (Tyr1217, 3871T), PLCγ2 (3872T), phospho-PLCγ1 (Tyr783, 2821T), PLCγ1 (5690T), c-Fos (4384), calcineurin A (2614S), phospho-p38 mitogen-activated protein kinase (MAPK) (Thr180/Tyr182, 9211), p38 MAPK (8690), phospho-SAPK/JNK (Thr183/Tyr185, 4668), SAPK/JNK (9252), phospho-ERK1/2 (4370), ERK1/2 (9102), phospho-nuclear factor-kappa B (NF-κB) p65 (Ser536, 3033), NF-κB p65 (8242), phospho-signal transducer and activator of transcription 5 (STAT5) (Tyr694, 9359S), STAT5 (94205T), phospho-STAT6 (Tyr641, 56554S), STAT6 (5397S), phospho-FAK (Tyr397, 3283S), and FAK (3285T) (all from Cell Signaling Technology, USA); TRAP (ab191406) and CXCR2 (ab217314; Abcam); CTSK (sc-48353), tumour necrosis factor receptor-associated factor 6 (TRAF6; sc-8409), and β-actin (sc-47778; Santa Cruz Biotech nology, USA).

    Article Title: Activating mutations in ESR1 contribute to an immunosuppressive breast tumor microenvironment by dampening cytokine secretion.
    Article Snippet: Flash-frozen mammary gland or tumor pieces were crushed in liquid nitrogen, and cells were collected into Eppendorf tubes by scraping and rinsed once in 1X PBS. .. The following antibodies were used for immunoblots: β-actin (Sigma, A5441, 1:2000), tubulin (Cell Signaling Technology (CST), 2148, 1:1000), Cyclin D1 (CST, 2922, 1:500), FOXA1 (Abcam, ab23738, 1:1000), Stat3 (CST, 9139, 1:1000), Stat5 (CST, 94205, 1:1000), p-Stat3 (CST, 9145, 1:1000), and p-Stat5 (CST, 4322, 1:500). ..

    Article Title: CXCL5 suppresses osteoclastogenesis and protects against lipoteichoic acid-induced bone loss by modulating PLCγ2 and c-Fos signalling in gram-positive periprosthetic joint infection
    Article Snippet: .. Western blotting was performed using primary antibodies against the following targets: nuclear factor of activated T-cells cytoplasmic 1 (NFATc1) (4389), phospho-phospholipase Cγ2 (PLCγ2) (Tyr1217, 3871T), PLCγ2 (3872T), phospho-PLCγ1 (Tyr783, 2821T), PLCγ1 (5690T), c-Fos (4384), calcineurin A (2614S), phospho-p38 mitogen-activated protein kinase (MAPK) (Thr180/Tyr182, 9211), p38 MAPK (8690), phospho-SAPK/JNK (Thr183/Tyr185, 4668), SAPK/JNK (9252), phospho-ERK1/2 (4370), ERK1/2 (9102), phospho-nuclear factor-kappa B (NF-κB) p65 (Ser536, 3033), NF-κB p65 (8242), phospho-signal transducer and activator of transcription 5 (STAT5) (Tyr694, 9359S), STAT5 (94205T), phospho-STAT6 (Tyr641, 56554S), STAT6 (5397S), phospho-FAK (Tyr397, 3283S), and FAK (3285T) (all from Cell Signaling Technology, USA); TRAP (ab191406) and CXCR2 (ab217314; Abcam); CTSK (sc-48353), tumour necrosis factor receptor-associated factor 6 (TRAF6; sc-8409), and β-actin (sc-47778; Santa Cruz Biotechnology, USA). .. Signal detection was carried out using enhanced chemiluminescence solution (Revvity, USA) and visualized with a UVP imaging system (Analytik Jena, Germany).

    Cell-Signaling:

    Article Title: CXCL5 suppresses osteoclastogenesis and protects against lipoteichoic acid-induced bone loss by modulating PLCγ2 and c-Fos signalling in gram-positive periprosthetic joint infection
    Article Snippet: .. Western blotting was performed using primary antibodies against the following targets: nuclear factor of activated T-cells cytoplasmic 1 (NFATc1) (4389), phospho-phospholipase Cγ2 (PLCγ2) (Tyr1217, 3871T), PLCγ2 (3872T), phospho-PLCγ1 (Tyr783, 2821T), PLCγ1 (5690T), c-Fos (4384), calcineurin A (2614S), phospho-p38 mitogen-activated protein kinase (MAPK) (Thr180/Tyr182, 9211), p38 MAPK (8690), phospho-SAPK/JNK (Thr183/Tyr185, 4668), SAPK/JNK (9252), phospho-ERK1/2 (4370), ERK1/2 (9102), phospho-nuclear factor-kappa B (NF-κB) p65 (Ser536, 3033), NF-κB p65 (8242), phospho-signal transducer and activator of transcription 5 (STAT5) (Tyr694, 9359S), STAT5 (94205T), phospho-STAT6 (Tyr641, 56554S), STAT6 (5397S), phospho-FAK (Tyr397, 3283S), and FAK (3285T) (all from Cell Signaling Technology, USA); TRAP (ab191406) and CXCR2 (ab217314; Abcam); CTSK (sc-48353), tumour necrosis factor receptor-associated factor 6 (TRAF6; sc-8409), and β-actin (sc-47778; Santa Cruz Biotechnology, USA). .. Signal detection was carried out using enhanced chemiluminescence solution (Revvity, USA) and visualized with a UVP imaging system (Analytik Jena, Germany).

    other:

    Article Title: Homoharringtonine and Gilteritinib Synergistically Induce Apoptosis and Suppress Viability in FLT3-ITD-Positive AML Cells.
    Article Snippet: Antibodies against FLT3 (#3462), p-FLT3 (Tyr589/591, #3464), p-STAT5 (Tyr694, #4322), STAT5 (#94205), AKT (#4691), p-AKT (Ser473, #4060), ERK (#4695), p-ERK (Thr202/Tyr204, #4370), p21 (#29477), HSPA8 (#8444), anti-rabbit IgG (#7074), anti-mouse IgG (#7076), and GAPDH (#8884) were obtained from Cell Signaling Technology (Danvers, MA, USA).

    Article Title: Activating mutations in ESR1 contribute to an immunosuppressive breast tumor microenvironment by dampening cytokine secretion
    Article Snippet: Band intensity quantification was done using Image Studio Lite software (Li-Cor).

    Expressing:

    Article Title: Combining lenalidomide with IL-2 family of cytokines enhances activating receptor and perforin/granzyme expression in NK cells
    Article Snippet: After assessing that the highest band transferred completely membranes were retrieved and blocked with 5% nonfat milk-based tris buffer with 0.05% Tween 20 (cat# BP337−500, Thermo Fisher Scientific, Waltham, Massachusetts, United States). .. The primary antibodies used for protein expression assessment were: total STAT5 (D3N2B, 1:1000, cat# 25656, Cell Signaling Technology, Danvers, Massachusetts, United States, RRID:AB_2798908), phospho- STAT5 (C11C5, 1:1000, cat# 9359P, Cell Signaling Technology, Danvers, Massachusetts, United States), total AKT (11E7, 1:1000, cat# 4685S, Cell Signaling Technology, Danvers, Massachusetts, United States), phospho-AKT (D25E6, 1:1000, cat# 130385, Cell Signaling Technology, Danvers, Massachusetts, United States), PI3K p85 (I9H8, 1:1000, cat# 4257, Cell Signaling Technology, Danvers, Massachusetts, United States, RRID:AB_659889) and beta-actin as a loading control (AC-74, 1:5000, cat#A5316, Sigma-Aldrich , St. Louis, Missouri, United States, RRID:AB_476743). ..

    Article Title: Combining lenalidomide with IL-2 family of cytokines enhances activating receptor and perforin/granzyme expression in NK cells.
    Article Snippet: After assessing that the highest band transferred completely membranes were retrieved and blocked with 5% nonfat milk-based tris buffer with 0.05% Tween 20 (cat# BP337−500, Thermo Fisher Scientific, Waltham, Massachusetts, United States). .. The primary antibodies used for protein expression assessment were: total STAT5 (D3N2B, 1:1000, cat# 25656, Cell Signaling Technology, Danvers, Massachusetts, United States, RRID:AB_2798908), phospho- STAT5 (C11C5, 1:1000, cat# 9359P, Cell Signaling Technology, Danvers, Massachusetts, United States), total AKT (11E7, 1:1000, cat# 4685S, Cell Signaling Technology, Danvers, Massachusetts, United States), phospho-AKT (D25E6, 1:1000, cat# 130385, Cell Signaling Technology, Danvers, Massachusetts, United States), PI3K p85 (I9H8, 1:1000, cat# 4257, Cell Signaling Technology, Danvers, Massachusetts, United States, RRID:AB_659889) and beta-actin as a loading control (AC-74, 1:5000, cat#A5316, Sigma-Aldrich, St. Louis, Missouri, United States, RRID:AB_476743). ..

    Control:

    Article Title: Combining lenalidomide with IL-2 family of cytokines enhances activating receptor and perforin/granzyme expression in NK cells
    Article Snippet: After assessing that the highest band transferred completely membranes were retrieved and blocked with 5% nonfat milk-based tris buffer with 0.05% Tween 20 (cat# BP337−500, Thermo Fisher Scientific, Waltham, Massachusetts, United States). .. The primary antibodies used for protein expression assessment were: total STAT5 (D3N2B, 1:1000, cat# 25656, Cell Signaling Technology, Danvers, Massachusetts, United States, RRID:AB_2798908), phospho- STAT5 (C11C5, 1:1000, cat# 9359P, Cell Signaling Technology, Danvers, Massachusetts, United States), total AKT (11E7, 1:1000, cat# 4685S, Cell Signaling Technology, Danvers, Massachusetts, United States), phospho-AKT (D25E6, 1:1000, cat# 130385, Cell Signaling Technology, Danvers, Massachusetts, United States), PI3K p85 (I9H8, 1:1000, cat# 4257, Cell Signaling Technology, Danvers, Massachusetts, United States, RRID:AB_659889) and beta-actin as a loading control (AC-74, 1:5000, cat#A5316, Sigma-Aldrich , St. Louis, Missouri, United States, RRID:AB_476743). ..

    Article Title: Combining lenalidomide with IL-2 family of cytokines enhances activating receptor and perforin/granzyme expression in NK cells.
    Article Snippet: After assessing that the highest band transferred completely membranes were retrieved and blocked with 5% nonfat milk-based tris buffer with 0.05% Tween 20 (cat# BP337−500, Thermo Fisher Scientific, Waltham, Massachusetts, United States). .. The primary antibodies used for protein expression assessment were: total STAT5 (D3N2B, 1:1000, cat# 25656, Cell Signaling Technology, Danvers, Massachusetts, United States, RRID:AB_2798908), phospho- STAT5 (C11C5, 1:1000, cat# 9359P, Cell Signaling Technology, Danvers, Massachusetts, United States), total AKT (11E7, 1:1000, cat# 4685S, Cell Signaling Technology, Danvers, Massachusetts, United States), phospho-AKT (D25E6, 1:1000, cat# 130385, Cell Signaling Technology, Danvers, Massachusetts, United States), PI3K p85 (I9H8, 1:1000, cat# 4257, Cell Signaling Technology, Danvers, Massachusetts, United States, RRID:AB_659889) and beta-actin as a loading control (AC-74, 1:5000, cat#A5316, Sigma-Aldrich, St. Louis, Missouri, United States, RRID:AB_476743). ..



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